multi dna fragment assembly (New England Biolabs)
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multi dna fragment assembly
Multi Dna Fragment Assembly, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 9343 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multi+dna+fragment+assembly/NEBuilder+HiFi+DNA+Assembly+Master+Mix/pm36562145-338-30-35
Average 99 stars, based on 9343 article reviews
Multi Dna Fragment Assembly, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 9343 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multi+dna+fragment+assembly/NEBuilder+HiFi+DNA+Assembly+Master+Mix/pm36562145-338-30-35
Average 99 stars, based on 9343 article reviews
multi dna fragment assembly - by Bioz Stars,
2026-10
99/100 stars
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Plasmid Preparation:Article Title: F-actin regulates the polarized secretion of pollen tube attractants in Arabidopsis synergid cells. Article Snippet: Plasma membrane markers were constructed as follows. pOR084, an entry clone containing mRUBY2-SYP132, was constructed using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, MA, USA) by multi-DNA fragment assembly between (i) mRUBY2-containing pENTR/D-TOPO (Thermo Fisher Scientific, MA, USA) plasmid backbone amplified by a primer set “FP_SpeI_F” and “FP_EcoRI_R” and (ii) SYP132 (At5g08080) cDNA sequence amplified by a primer set “SYP132_EcoRI_F” and “SYP132_SpeI_R”. pENTR/D-TOPO plasmid backbone with SYP132 was amplified from pOR084 by PCR using primers “GFP_1-18_R” and “XFP_C-term_F”. .. The resulting linearized plasmid DNA was mixed with three mNeonGreen DNA fragments independently amplified using the “GFP F” and “Linker1_XFP_R”, “Linker1_XFP_F” and “Linker2_XFP_R”, or “Linker2_XFP_F” and “FP_R” primer sets, and Article Title: F-actin regulates polarized secretion of pollen tube attractants in Arabidopsis synergid cell Article Snippet: The pOR084, an entry clone containing mRUBY2-SYP132 , was constructed using NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, MA, USA) by multi-DNA fragment assembly between (i) mRUBY2 -containing pENTR/D-TOPO (Thermo Fisher Scientific, MA, USA) plasmid backbone amplified by a primer set ‘FP_SpeI_F’ and ‘FP_EcoRI_R’ and (ii) SYP132 (At5g08080) cDNA sequence amplified by a primer set ‘SYP132_EcoRI_F’ and ‘SYP132_SpeI_R’. pENTR/D-TOPO plasmid backbone with the SYP132 was amplified from the pOR084 by a PCR using primers ‘GFP_1-18_R’ and ‘XFP_C-term_F’. .. Resulting linearized plasmid DNA was mixed with three mNeonGreen DNA fragments independently amplified by the following three primer sets, ‘GFP F’ and ‘Linker1_XFP_R’, ‘Linker1_XFP_F’ and ‘Linker2_XFP_R’, or ‘Linker2_XFP_F’ and ‘FP_R’, and Amplification:Article Title: F-actin regulates the polarized secretion of pollen tube attractants in Arabidopsis synergid cells. Article Snippet: Plasma membrane markers were constructed as follows. pOR084, an entry clone containing mRUBY2-SYP132, was constructed using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, MA, USA) by multi-DNA fragment assembly between (i) mRUBY2-containing pENTR/D-TOPO (Thermo Fisher Scientific, MA, USA) plasmid backbone amplified by a primer set “FP_SpeI_F” and “FP_EcoRI_R” and (ii) SYP132 (At5g08080) cDNA sequence amplified by a primer set “SYP132_EcoRI_F” and “SYP132_SpeI_R”. pENTR/D-TOPO plasmid backbone with SYP132 was amplified from pOR084 by PCR using primers “GFP_1-18_R” and “XFP_C-term_F”. .. The resulting linearized plasmid DNA was mixed with three mNeonGreen DNA fragments independently amplified using the “GFP F” and “Linker1_XFP_R”, “Linker1_XFP_F” and “Linker2_XFP_R”, or “Linker2_XFP_F” and “FP_R” primer sets, and Article Title: F-actin regulates polarized secretion of pollen tube attractants in Arabidopsis synergid cell Article Snippet: The pOR084, an entry clone containing mRUBY2-SYP132 , was constructed using NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, MA, USA) by multi-DNA fragment assembly between (i) mRUBY2 -containing pENTR/D-TOPO (Thermo Fisher Scientific, MA, USA) plasmid backbone amplified by a primer set ‘FP_SpeI_F’ and ‘FP_EcoRI_R’ and (ii) SYP132 (At5g08080) cDNA sequence amplified by a primer set ‘SYP132_EcoRI_F’ and ‘SYP132_SpeI_R’. pENTR/D-TOPO plasmid backbone with the SYP132 was amplified from the pOR084 by a PCR using primers ‘GFP_1-18_R’ and ‘XFP_C-term_F’. .. Resulting linearized plasmid DNA was mixed with three mNeonGreen DNA fragments independently amplified by the following three primer sets, ‘GFP F’ and ‘Linker1_XFP_R’, ‘Linker1_XFP_F’ and ‘Linker2_XFP_R’, or ‘Linker2_XFP_F’ and ‘FP_R’, and Cloning:Article Title: F-actin regulates the polarized secretion of pollen tube attractants in Arabidopsis synergid cells. Article Snippet: Plasma membrane markers were constructed as follows. pOR084, an entry clone containing mRUBY2-SYP132, was constructed using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, MA, USA) by multi-DNA fragment assembly between (i) mRUBY2-containing pENTR/D-TOPO (Thermo Fisher Scientific, MA, USA) plasmid backbone amplified by a primer set “FP_SpeI_F” and “FP_EcoRI_R” and (ii) SYP132 (At5g08080) cDNA sequence amplified by a primer set “SYP132_EcoRI_F” and “SYP132_SpeI_R”. pENTR/D-TOPO plasmid backbone with SYP132 was amplified from pOR084 by PCR using primers “GFP_1-18_R” and “XFP_C-term_F”. .. The resulting linearized plasmid DNA was mixed with three mNeonGreen DNA fragments independently amplified using the “GFP F” and “Linker1_XFP_R”, “Linker1_XFP_F” and “Linker2_XFP_R”, or “Linker2_XFP_F” and “FP_R” primer sets, and Article Title: F-actin regulates polarized secretion of pollen tube attractants in Arabidopsis synergid cell Article Snippet: The pOR084, an entry clone containing mRUBY2-SYP132 , was constructed using NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, MA, USA) by multi-DNA fragment assembly between (i) mRUBY2 -containing pENTR/D-TOPO (Thermo Fisher Scientific, MA, USA) plasmid backbone amplified by a primer set ‘FP_SpeI_F’ and ‘FP_EcoRI_R’ and (ii) SYP132 (At5g08080) cDNA sequence amplified by a primer set ‘SYP132_EcoRI_F’ and ‘SYP132_SpeI_R’. pENTR/D-TOPO plasmid backbone with the SYP132 was amplified from the pOR084 by a PCR using primers ‘GFP_1-18_R’ and ‘XFP_C-term_F’. .. Resulting linearized plasmid DNA was mixed with three mNeonGreen DNA fragments independently amplified by the following three primer sets, ‘GFP F’ and ‘Linker1_XFP_R’, ‘Linker1_XFP_F’ and ‘Linker2_XFP_R’, or ‘Linker2_XFP_F’ and ‘FP_R’, and |